Tailoring the chemical functionalization of a transparent polyethylene foil for its application in an OLED-based DNA biosensor
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In: Applied surface science, Vol. 552.2021, No. 30 June, 149408, 30.06.2021.
Research output: Contribution to journal › Article › Research › peer-review
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T1 - Tailoring the chemical functionalization of a transparent polyethylene foil for its application in an OLED-based DNA biosensor
AU - Daniso, Enrico
AU - Maroh, Boris
AU - Feldbacher, Sonja
AU - Mühlbacher, Inge
AU - Schlögl, Sandra
AU - Melpignano, Patrizia
PY - 2021/6/30
Y1 - 2021/6/30
N2 - For the development of a bio-sensor based on fluorescence excitation in transmission, a plastic substrate with excellent optical characteristics, such as high optical transmission in the visible range and low self-fluorescence, is required. Polyethylene (PE) films have been found to meet these optical characteristics. However, the adhesion of short sequences of oligonucleotides used as bio-probes, which are needed to develop a genosensor, has not proven ideal on this substrate, due to its olefinic composition. In order to overcome this challenge, a physical-chemical surface modification of PE films has been performed using a corona treatment, followed by the attachment of functional organic compounds. The surface modified substrates have been characterized for their wettability, surface energy, zeta potential and surface topography. The bio-probes’ adhesion on the treated surfaces has been tested by monitoring the fluorescence of dye-conjugated complementary sequences of the oligonucleotides deposited on the modified PE substrates. Finally, two modified PE substrates, comprising the best adhesion of the bio-probes, have been used to prepare a genosensor for the detection of the DNA of the insect Hermetia illucens. As expected, the better adhesion of bio-probes on the functionalized PE substrate has allowed a significant improvement of the bio-sensor’s limit of detection (LOD).
AB - For the development of a bio-sensor based on fluorescence excitation in transmission, a plastic substrate with excellent optical characteristics, such as high optical transmission in the visible range and low self-fluorescence, is required. Polyethylene (PE) films have been found to meet these optical characteristics. However, the adhesion of short sequences of oligonucleotides used as bio-probes, which are needed to develop a genosensor, has not proven ideal on this substrate, due to its olefinic composition. In order to overcome this challenge, a physical-chemical surface modification of PE films has been performed using a corona treatment, followed by the attachment of functional organic compounds. The surface modified substrates have been characterized for their wettability, surface energy, zeta potential and surface topography. The bio-probes’ adhesion on the treated surfaces has been tested by monitoring the fluorescence of dye-conjugated complementary sequences of the oligonucleotides deposited on the modified PE substrates. Finally, two modified PE substrates, comprising the best adhesion of the bio-probes, have been used to prepare a genosensor for the detection of the DNA of the insect Hermetia illucens. As expected, the better adhesion of bio-probes on the functionalized PE substrate has allowed a significant improvement of the bio-sensor’s limit of detection (LOD).
KW - Fluorescence detection
KW - Genosensor
KW - Oligonucleotide bio-probe
KW - Polymeric surface modification
UR - http://www.scopus.com/inward/record.url?scp=85102342489&partnerID=8YFLogxK
U2 - 10.1016/j.apsusc.2021.149408
DO - 10.1016/j.apsusc.2021.149408
M3 - Article
AN - SCOPUS:85102342489
VL - 552.2021
JO - Applied surface science
JF - Applied surface science
SN - 0169-4332
IS - 30 June
M1 - 149408
ER -