Tailoring the chemical functionalization of a transparent polyethylene foil for its application in an OLED-based DNA biosensor

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Tailoring the chemical functionalization of a transparent polyethylene foil for its application in an OLED-based DNA biosensor. / Daniso, Enrico; Maroh, Boris; Feldbacher, Sonja et al.
In: Applied surface science, Vol. 552.2021, No. 30 June, 149408, 30.06.2021.

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Daniso E, Maroh B, Feldbacher S, Mühlbacher I, Schlögl S, Melpignano P. Tailoring the chemical functionalization of a transparent polyethylene foil for its application in an OLED-based DNA biosensor. Applied surface science. 2021 Jun 30;552.2021(30 June):149408. Epub 2021 Mar 10. doi: 10.1016/j.apsusc.2021.149408

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Daniso, Enrico ; Maroh, Boris ; Feldbacher, Sonja et al. / Tailoring the chemical functionalization of a transparent polyethylene foil for its application in an OLED-based DNA biosensor. In: Applied surface science. 2021 ; Vol. 552.2021, No. 30 June.

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@article{7d2b10935de3490e9ac957d26c38e987,
title = "Tailoring the chemical functionalization of a transparent polyethylene foil for its application in an OLED-based DNA biosensor",
abstract = "For the development of a bio-sensor based on fluorescence excitation in transmission, a plastic substrate with excellent optical characteristics, such as high optical transmission in the visible range and low self-fluorescence, is required. Polyethylene (PE) films have been found to meet these optical characteristics. However, the adhesion of short sequences of oligonucleotides used as bio-probes, which are needed to develop a genosensor, has not proven ideal on this substrate, due to its olefinic composition. In order to overcome this challenge, a physical-chemical surface modification of PE films has been performed using a corona treatment, followed by the attachment of functional organic compounds. The surface modified substrates have been characterized for their wettability, surface energy, zeta potential and surface topography. The bio-probes{\textquoteright} adhesion on the treated surfaces has been tested by monitoring the fluorescence of dye-conjugated complementary sequences of the oligonucleotides deposited on the modified PE substrates. Finally, two modified PE substrates, comprising the best adhesion of the bio-probes, have been used to prepare a genosensor for the detection of the DNA of the insect Hermetia illucens. As expected, the better adhesion of bio-probes on the functionalized PE substrate has allowed a significant improvement of the bio-sensor{\textquoteright}s limit of detection (LOD).",
keywords = "Fluorescence detection, Genosensor, Oligonucleotide bio-probe, Polymeric surface modification",
author = "Enrico Daniso and Boris Maroh and Sonja Feldbacher and Inge M{\"u}hlbacher and Sandra Schl{\"o}gl and Patrizia Melpignano",
year = "2021",
month = jun,
day = "30",
doi = "10.1016/j.apsusc.2021.149408",
language = "English",
volume = "552.2021",
journal = "Applied surface science",
issn = "0169-4332",
publisher = "Elsevier",
number = "30 June",

}

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TY - JOUR

T1 - Tailoring the chemical functionalization of a transparent polyethylene foil for its application in an OLED-based DNA biosensor

AU - Daniso, Enrico

AU - Maroh, Boris

AU - Feldbacher, Sonja

AU - Mühlbacher, Inge

AU - Schlögl, Sandra

AU - Melpignano, Patrizia

PY - 2021/6/30

Y1 - 2021/6/30

N2 - For the development of a bio-sensor based on fluorescence excitation in transmission, a plastic substrate with excellent optical characteristics, such as high optical transmission in the visible range and low self-fluorescence, is required. Polyethylene (PE) films have been found to meet these optical characteristics. However, the adhesion of short sequences of oligonucleotides used as bio-probes, which are needed to develop a genosensor, has not proven ideal on this substrate, due to its olefinic composition. In order to overcome this challenge, a physical-chemical surface modification of PE films has been performed using a corona treatment, followed by the attachment of functional organic compounds. The surface modified substrates have been characterized for their wettability, surface energy, zeta potential and surface topography. The bio-probes’ adhesion on the treated surfaces has been tested by monitoring the fluorescence of dye-conjugated complementary sequences of the oligonucleotides deposited on the modified PE substrates. Finally, two modified PE substrates, comprising the best adhesion of the bio-probes, have been used to prepare a genosensor for the detection of the DNA of the insect Hermetia illucens. As expected, the better adhesion of bio-probes on the functionalized PE substrate has allowed a significant improvement of the bio-sensor’s limit of detection (LOD).

AB - For the development of a bio-sensor based on fluorescence excitation in transmission, a plastic substrate with excellent optical characteristics, such as high optical transmission in the visible range and low self-fluorescence, is required. Polyethylene (PE) films have been found to meet these optical characteristics. However, the adhesion of short sequences of oligonucleotides used as bio-probes, which are needed to develop a genosensor, has not proven ideal on this substrate, due to its olefinic composition. In order to overcome this challenge, a physical-chemical surface modification of PE films has been performed using a corona treatment, followed by the attachment of functional organic compounds. The surface modified substrates have been characterized for their wettability, surface energy, zeta potential and surface topography. The bio-probes’ adhesion on the treated surfaces has been tested by monitoring the fluorescence of dye-conjugated complementary sequences of the oligonucleotides deposited on the modified PE substrates. Finally, two modified PE substrates, comprising the best adhesion of the bio-probes, have been used to prepare a genosensor for the detection of the DNA of the insect Hermetia illucens. As expected, the better adhesion of bio-probes on the functionalized PE substrate has allowed a significant improvement of the bio-sensor’s limit of detection (LOD).

KW - Fluorescence detection

KW - Genosensor

KW - Oligonucleotide bio-probe

KW - Polymeric surface modification

UR - http://www.scopus.com/inward/record.url?scp=85102342489&partnerID=8YFLogxK

U2 - 10.1016/j.apsusc.2021.149408

DO - 10.1016/j.apsusc.2021.149408

M3 - Article

AN - SCOPUS:85102342489

VL - 552.2021

JO - Applied surface science

JF - Applied surface science

SN - 0169-4332

IS - 30 June

M1 - 149408

ER -