The Middle Miocene Balikpapan Formation is exposed in the Samarinda Anticlinorium, which forms part of the Lower Kutai Basin situated on East Kalimantan, Indonesia. The Balikpapan Formation is considered the main source rock for oil and gas fields in Lower Kutai Basin. This study integrates sedimentology, organic geochemistry, organic petrography and calcareous nannofossil analysis to characterize the depositional environment, to determine the source of the organic matter, and to assess the hydrocarbon potential of the Balikpapan Formation. The studied sections contain at one locality calcareous nannofossil assemblages with low diversity including Sphenolithus heteromorphus, suggesting nannofossil zones NN4 – NN5 (upper Burdigalian - Langhian-lower Serravallian) are restricted to the Air Putih section in the northeastern part of the study area. Six facies associations were identified in the study area, comprising eleven lithofacies, interpreted as fluvial-deltaic to shallow marine in origin. The fine-grained lithofacies include shale, coaly shale and coal while the coarse-grained facies include sandstone and sandy conglomerates. The geochemical results (TOC) indicate that the analyzed samples have strongly varying total organic carbon (TOC) contents. The organic matter is composed of type III (gas-prone) and type II-III (mixed oil and gas prone) kerogen, with HI values ranging from 32 to 252 mg HC/g TOC. The Rock-Eval parameter Tmax 409–441 °C and vitrinite reflectance values (0.40–0.67 %Rr) indicate that the sediments are immature to marginal mature. The rank of coal in the Balikpapan Formation ranges from the sub-bituminous to the high-volatile bituminous B stage.