Pteris vittata (PV) and Pteris quadriaurita (PQ) are reported to hyperaccumulate arsenic (As) when grown in As-rich soil. Yet, little is known about the impact of their unique As accumulation mechanisms on As transformations and cycling at the soil-root interface. Using a combined approach of two-dimensional (2D), sub-mm scale solute imaging of arsenite (As ^III), arsenate (As ^V), phosphorus (P), manganese (Mn), iron (Fe) and oxygen (O ₂), we found localized patterns of As ^III/As ^V redox transformations in the PV rhizosphere (As ^III/As ^V ratio of 0.57) compared to bulk soil (As ^III/As ^V ratio of ≤0.04). Our data indicate that the high As root uptake, translocation and accumulation from the As-rich experimental soil (2080 mg kg ⁻¹) to PV fronds (6986 mg kg ⁻¹) induced As detoxification via As ^V reduction and As ^III root efflux, leading to As ^III accumulation and re-oxidation to As ^V in the rhizosphere porewater. This As cycling mechanism is linked to the reduction of O ₂ and Mn ^III/IV (oxyhydr)oxides resulting in decreased O ₂ levels and increased Mn solubilization along roots. Compared to PV, we found 4-fold lower As translocation to PQ fronds (1611 mg kg ⁻¹), 2-fold lower As ^V depletion in the PQ rhizosphere, and no As ^III efflux from PQ roots, suggesting that PQ efficiently controls As uptake to avoid toxic As levels in roots. Analysis of root exudates obtained from soil-grown PV showed that As acquisition by PV roots was not associated with phytic acid release. Our study demonstrates that two closely-related As-accumulating ferns have distinct mechanisms for As uptake modulating As cycling in As-rich environments.