Bio-based polymers have better salt and temperature tolerance than most synthetic polymers. The biopolymer solutions have high viscosity, which can lead to reducing the fingering effect and soaring the oil recovery rate. This work aims to produce and characterize a biopolymer from Pseudomonas Atacamensis M7D1 strain, modify the biopolymer yield using Printed Circuit Boards (PCBs) powder as an outer tension in the growth medium, and finally, evaluate the produced biopolymer function for Enhanced Oil Recovery (EOR) purposes. Using PCBs powder to trigger bacteria for higher production yield increases the biopolymer production rate eleven times higher than pure growth medium without additives. Different analyses were performed on the biopolymer to characterize its properties; Gel Permeation Chromatography (GPC) indicated that the produced biopolymer has an average molecular weight of 3.6 × 105 g/mol. This macromolecule has high thermal resistivity and can tolerate high temperatures. Thermal analysis (TGA/DSC) shows only 69.27 % mass lost from 25 ◦C to 500 ◦C. The viscosity of 0.5 wt% biopolymer solution equals 3cp, 3 times higher than water. The glass micromodel flooding result shows that biopolymer solution with 0.5 wt% concentration has a 42 % recovery rate which is 24 % higher
than water flooding.