TY - JOUR

T1 - Enriched stable 204Pb as tracer at ultra-low levels in clinical investigations

AU - Irrgeher, Johanna

AU - Berger, Thomas

AU - Tchaikovsky, Anastassiya

AU - Tschegg, Cornelius

AU - Gouya, Ghazaleh

AU - Lechner, Peter

AU - Retzmann, Anika

AU - Opper, Christine

AU - Firbas, Christa

AU - Freissmuth, Michael

AU - Peschel-Credner, Kerstin

AU - Anderle, Karolina

AU - Meisslitzer, Claudia

AU - Wolzt, Michael

AU - Prohaska, Thomas

N1 - Publisher Copyright: © 2022, The Author(s).

PY - 2022/9/22

Y1 - 2022/9/22

N2 - The potential of enriched Pb (204Pb) was assessed to monitor pathways of trace levels of Pb in the pg range within the human body via isotope pattern variation in situations where natural lead cannot be used as a tracer due to regulatory limitations. Isotope ratio measurements were accomplished by means of (multi-collector) inductively coupled plasma mass spectrometry including a comparison of single and multi-collector ICP-MS for low-level 204Pb assessment. Isotopic pattern results from a blend of a large quantity of the element with a natural isotopic composition and an enriched stable isotope at orders of magnitude lower levels pose a nontrivial analytical problem. Isotope pattern deconvolution was successfully applied as mathematical tool based on multiple linear regressions. The method allowed for deconvolving the isotope pattern from measured isotope ratios without knowing the quantities of different isotope sources incorporated and mixed into the sample at levels of < 1 pg 204Pb/g blood. The objective of this manuscript is to evaluate and summarize the analytical aspects for Pb isotope pattern deconvolution based on the results of a clinical trial, where a 204Pb-enriched isotope tracer was applied to investigate the bioavailability of orally applied Pb along with purified clinoptilolite tuff as potential supplement. This unique approach allows to reduce tracer amounts to harmless levels to human health, which are in accordance with the legal regulative to study enrichment levels of < 0.01% in human blood.

AB - The potential of enriched Pb (204Pb) was assessed to monitor pathways of trace levels of Pb in the pg range within the human body via isotope pattern variation in situations where natural lead cannot be used as a tracer due to regulatory limitations. Isotope ratio measurements were accomplished by means of (multi-collector) inductively coupled plasma mass spectrometry including a comparison of single and multi-collector ICP-MS for low-level 204Pb assessment. Isotopic pattern results from a blend of a large quantity of the element with a natural isotopic composition and an enriched stable isotope at orders of magnitude lower levels pose a nontrivial analytical problem. Isotope pattern deconvolution was successfully applied as mathematical tool based on multiple linear regressions. The method allowed for deconvolving the isotope pattern from measured isotope ratios without knowing the quantities of different isotope sources incorporated and mixed into the sample at levels of < 1 pg 204Pb/g blood. The objective of this manuscript is to evaluate and summarize the analytical aspects for Pb isotope pattern deconvolution based on the results of a clinical trial, where a 204Pb-enriched isotope tracer was applied to investigate the bioavailability of orally applied Pb along with purified clinoptilolite tuff as potential supplement. This unique approach allows to reduce tracer amounts to harmless levels to human health, which are in accordance with the legal regulative to study enrichment levels of < 0.01% in human blood.

KW - Blood

KW - Zeolite

KW - Lead

KW - Clinoptilolite

KW - Isotope pattern deconvolution

KW - ICP-MS

UR - http://www.scopus.com/inward/record.url?scp=85138570639&partnerID=8YFLogxK

U2 - 10.1007/s00216-022-04311-0

DO - 10.1007/s00216-022-04311-0

M3 - Article

VL - 415.2023

SP - 255

EP - 268

JO - Analytical and bioanalytical chemistry

JF - Analytical and bioanalytical chemistry

SN - 1618-2642

IS - January

ER -