Biomass (especially algae) is a renewable energy source that can be a great alternative to fossil fuels. Wet algal biomass converts into products such as solid, aqueous, and gaseous phases as well as biocrude in hydrothermal liquefaction (HTL). The aim of this work was to provide detailed exergy analyses of the production of biocrude from Nannochloropsis sp. by HTL. Physical and chemical exergy of the HTL products, exergy losses, exergy efficiency, and exergy distribution of the HTL process were determined in this research. The highest exergy loss and the lowest efficiency values obtained for the heat exchanger were 65,856.83 MJ/hr and 66.64%, respectively, which was mainly caused by the irreversibility of the heat transfer process. Moreover, the HTL reactor had high efficiency (99.9%) due to the complex reactions that occurred at high temperature and pressure. Also, the optimum operating conditions of the reactor were obtained at 350 °C and 20 MPa by using sensitivity analysis. The high overall exergy efficiency of the process (94.93%) indicated that HTL was the most effective process for the conversion of algae. In addition, the exergy recovery values of the overall exergy input values in the HTL process for biocrude, as well as the aqueous, solid, and gas phases, were nearly 74.88%, 18.42%, 0.86%, and 0.76%, respectively. Exergy assessment provides beneficial information for improving the thermodynamic performance of the HTL system.