Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases-application to feed supplements

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Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases-application to feed supplements. / Neubauer, Stefan; Rugova, Ariana; Chu, Dinh Binh et al.
In: Analytical and bioanalytical chemistry, Vol. 404.2012, No. 3, 08.2012, p. 799-808.

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Neubauer S, Rugova A, Chu DB, Drexler H, Ganner A, Sauer M et al. Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases-application to feed supplements. Analytical and bioanalytical chemistry. 2012 Aug;404.2012(3):799-808. doi: 10.1007/s00216-012-6170-9

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Neubauer, Stefan ; Rugova, Ariana ; Chu, Dinh Binh et al. / Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases-application to feed supplements. In: Analytical and bioanalytical chemistry. 2012 ; Vol. 404.2012, No. 3. pp. 799-808.

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@article{c08a89a5ffe84764956db6124a43058b,
title = "Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases-application to feed supplements",
abstract = "In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100 % aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection <1 μmol L1 could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.",
keywords = "Feed supplemen, LC-ICP-MS, LC-MS-MS, Nucleobase, Nucleoside, Nucleotide",
author = "Stefan Neubauer and Ariana Rugova and Chu, {Dinh Binh} and Hedda Drexler and Anja Ganner and Michael Sauer and Diethard Mattanovich and Stephan Hann and Gunda Koellensperger",
note = "Funding Information: Acknowledgements This work was supported by the Biomin B.R.A.I.N program and by the Austrian Research Promotion Agency FFG (FHplus projekt METORGANIC).",
year = "2012",
month = aug,
doi = "10.1007/s00216-012-6170-9",
language = "English",
volume = "404.2012",
pages = "799--808",
journal = "Analytical and bioanalytical chemistry",
issn = "1618-2642",
publisher = "Springer Berlin",
number = "3",

}

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TY - JOUR

T1 - Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases-application to feed supplements

AU - Neubauer, Stefan

AU - Rugova, Ariana

AU - Chu, Dinh Binh

AU - Drexler, Hedda

AU - Ganner, Anja

AU - Sauer, Michael

AU - Mattanovich, Diethard

AU - Hann, Stephan

AU - Koellensperger, Gunda

N1 - Funding Information: Acknowledgements This work was supported by the Biomin B.R.A.I.N program and by the Austrian Research Promotion Agency FFG (FHplus projekt METORGANIC).

PY - 2012/8

Y1 - 2012/8

N2 - In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100 % aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection <1 μmol L1 could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.

AB - In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100 % aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection <1 μmol L1 could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.

KW - Feed supplemen

KW - LC-ICP-MS

KW - LC-MS-MS

KW - Nucleobase

KW - Nucleoside

KW - Nucleotide

UR - http://www.scopus.com/inward/record.url?scp=84865497284&partnerID=8YFLogxK

U2 - 10.1007/s00216-012-6170-9

DO - 10.1007/s00216-012-6170-9

M3 - Article

C2 - 22752403

AN - SCOPUS:84865497284

VL - 404.2012

SP - 799

EP - 808

JO - Analytical and bioanalytical chemistry

JF - Analytical and bioanalytical chemistry

SN - 1618-2642

IS - 3

ER -