Fungicide application increased copper-bioavailability and impaired nitrogen fixation through reduced root nodule formation on alfalfa

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Fungicide application increased copper-bioavailability and impaired nitrogen fixation through reduced root nodule formation on alfalfa. / Schneider, Martin; Keiblinger, Katharina M.; Paumann, Melanie et al.
In: Ecotoxicology, Vol. 28.2019, No. 6, 28.05.2019, p. 599-611.

Research output: Contribution to journalArticleResearchpeer-review

Harvard

Schneider, M, Keiblinger, KM, Paumann, M, Soja, G, Mentler, A, Golestani-Fard, A, Retzmann, A, Prohaska, T, Zechmeister-Boltenstern, S, Wenzel, W & Zehetner, F 2019, 'Fungicide application increased copper-bioavailability and impaired nitrogen fixation through reduced root nodule formation on alfalfa', Ecotoxicology, vol. 28.2019, no. 6, pp. 599-611. https://doi.org/10.1007/s10646-019-02047-9

APA

Schneider, M., Keiblinger, K. M., Paumann, M., Soja, G., Mentler, A., Golestani-Fard, A., Retzmann, A., Prohaska, T., Zechmeister-Boltenstern, S., Wenzel, W., & Zehetner, F. (2019). Fungicide application increased copper-bioavailability and impaired nitrogen fixation through reduced root nodule formation on alfalfa. Ecotoxicology, 28.2019(6), 599-611. Advance online publication. https://doi.org/10.1007/s10646-019-02047-9

Vancouver

Schneider M, Keiblinger KM, Paumann M, Soja G, Mentler A, Golestani-Fard A et al. Fungicide application increased copper-bioavailability and impaired nitrogen fixation through reduced root nodule formation on alfalfa. Ecotoxicology. 2019 May 28;28.2019(6):599-611. Epub 2019 May 28. doi: 10.1007/s10646-019-02047-9

Author

Schneider, Martin ; Keiblinger, Katharina M. ; Paumann, Melanie et al. / Fungicide application increased copper-bioavailability and impaired nitrogen fixation through reduced root nodule formation on alfalfa. In: Ecotoxicology. 2019 ; Vol. 28.2019, No. 6. pp. 599-611.

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@article{931aa4555137490f9e1b8246fe144b7a,
title = "Fungicide application increased copper-bioavailability and impaired nitrogen fixation through reduced root nodule formation on alfalfa",
abstract = "Copper-based fungicides have been used for a long time in viticulture and have accumulated in many vineyard soils. In this study, incrementing Cu(OH) 2-based fungicide application from 0.05 to 5 g Cu kg −1 on two agricultural soils (an acidic sandy loam (L, pH 4.95) and an alkaline silt loam (D, pH 7.45)) resulted in 5 times more mobile Cu in the acidic soil. The most sensitive parameters of alfalfa (Medicago sativa) growing in these soils were the root nodule number, decreasing to 34% and 15% of the control at 0.1 g Cu kg −1 in soil L and at 1.5 g Cu kg −1 in soil D, respectively, as well as the nodule biomass, decreasing to 25% and 27% at 0.5 g Cu kg −1 in soil L and at 1.5 g Cu kg −1 in soil D, respectively. However, the enzymatic N 2-fixation was not directly affected by Cu in spite of the presence of Cu in the meristem and the zone of effective N 2-fixation, as illustrated by chemical imaging. The strongly different responses observed in the two tested soils reflect the higher buffering capacity of the alkaline silt loam and showed that Cu mitigation and remediation strategies should especially target vineyards with acidic, sandy soils. ",
keywords = "Endosymbiosis, Heavy metal, LA-ICP-MS, Pollutant, Toxicology, Trace element",
author = "Martin Schneider and Keiblinger, {Katharina M.} and Melanie Paumann and Gerhard Soja and Axel Mentler and Alireza Golestani-Fard and Anika Retzmann and Thomas Prohaska and Sophie Zechmeister-Boltenstern and Walter Wenzel and Franz Zehetner",
note = "Publisher Copyright: {\textcopyright} 2019, The Author(s).",
year = "2019",
month = may,
day = "28",
doi = "10.1007/s10646-019-02047-9",
language = "English",
volume = "28.2019",
pages = "599--611",
journal = "Ecotoxicology",
issn = "0963-9292",
publisher = "Springer",
number = "6",

}

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TY - JOUR

T1 - Fungicide application increased copper-bioavailability and impaired nitrogen fixation through reduced root nodule formation on alfalfa

AU - Schneider, Martin

AU - Keiblinger, Katharina M.

AU - Paumann, Melanie

AU - Soja, Gerhard

AU - Mentler, Axel

AU - Golestani-Fard, Alireza

AU - Retzmann, Anika

AU - Prohaska, Thomas

AU - Zechmeister-Boltenstern, Sophie

AU - Wenzel, Walter

AU - Zehetner, Franz

N1 - Publisher Copyright: © 2019, The Author(s).

PY - 2019/5/28

Y1 - 2019/5/28

N2 - Copper-based fungicides have been used for a long time in viticulture and have accumulated in many vineyard soils. In this study, incrementing Cu(OH) 2-based fungicide application from 0.05 to 5 g Cu kg −1 on two agricultural soils (an acidic sandy loam (L, pH 4.95) and an alkaline silt loam (D, pH 7.45)) resulted in 5 times more mobile Cu in the acidic soil. The most sensitive parameters of alfalfa (Medicago sativa) growing in these soils were the root nodule number, decreasing to 34% and 15% of the control at 0.1 g Cu kg −1 in soil L and at 1.5 g Cu kg −1 in soil D, respectively, as well as the nodule biomass, decreasing to 25% and 27% at 0.5 g Cu kg −1 in soil L and at 1.5 g Cu kg −1 in soil D, respectively. However, the enzymatic N 2-fixation was not directly affected by Cu in spite of the presence of Cu in the meristem and the zone of effective N 2-fixation, as illustrated by chemical imaging. The strongly different responses observed in the two tested soils reflect the higher buffering capacity of the alkaline silt loam and showed that Cu mitigation and remediation strategies should especially target vineyards with acidic, sandy soils.

AB - Copper-based fungicides have been used for a long time in viticulture and have accumulated in many vineyard soils. In this study, incrementing Cu(OH) 2-based fungicide application from 0.05 to 5 g Cu kg −1 on two agricultural soils (an acidic sandy loam (L, pH 4.95) and an alkaline silt loam (D, pH 7.45)) resulted in 5 times more mobile Cu in the acidic soil. The most sensitive parameters of alfalfa (Medicago sativa) growing in these soils were the root nodule number, decreasing to 34% and 15% of the control at 0.1 g Cu kg −1 in soil L and at 1.5 g Cu kg −1 in soil D, respectively, as well as the nodule biomass, decreasing to 25% and 27% at 0.5 g Cu kg −1 in soil L and at 1.5 g Cu kg −1 in soil D, respectively. However, the enzymatic N 2-fixation was not directly affected by Cu in spite of the presence of Cu in the meristem and the zone of effective N 2-fixation, as illustrated by chemical imaging. The strongly different responses observed in the two tested soils reflect the higher buffering capacity of the alkaline silt loam and showed that Cu mitigation and remediation strategies should especially target vineyards with acidic, sandy soils.

KW - Endosymbiosis

KW - Heavy metal

KW - LA-ICP-MS

KW - Pollutant

KW - Toxicology

KW - Trace element

UR - http://www.scopus.com/inward/record.url?scp=85069227934&partnerID=8YFLogxK

U2 - 10.1007/s10646-019-02047-9

DO - 10.1007/s10646-019-02047-9

M3 - Article

VL - 28.2019

SP - 599

EP - 611

JO - Ecotoxicology

JF - Ecotoxicology

SN - 0963-9292

IS - 6

ER -