Quartz from the stockwork zone of various Cyprus type volcanogenic massive sulfide deposits (Boccassuolo, Reppia, Campegli, Bargone and Vigonzano) from the unmetamorphosed, Jurassic Northern Apennine ophiolites was studied in order to provide details on the submarine hydrothermal conditions and the characteristics for ore formation. Our detailed SEM-CL investigation of quartz contributed to a robust characterization and interpretation of primary fluid inclusions and microthermometry data. SEM-CL imaging was also useful for reconstructing the consecutive steps of quartz precipitation. The determination of trace element contents according to growth zon-ing in quartz by LA-ICP-MS constrained the compositional variations of parent fluids during the hydrothermal activity. A continuously cooling fluid regime characterized each studied volcano-genic massive sulfide (VMS) occurrence although the minimum formation temperatures were different (Bargone: 110–270 °C; Boccassuolo: 60–360 °C; Campegli: 110–225 °C; Reppia: 50–205 °C; Vigonzano: 260–330 °C), the range of temperature most probably depends on the original position of sampling in relation to the centers of the hydrothermal systems. Compositional changes are re-flected by variations in the methane content (0.13–0.33 mol/kg) and salinity (2.6–9.3 NaCl equiv. wt. %) in the fluid inclusions of quartz and calcite as well as a changeable Al content (11–1526 ppm) in quartz. This study demonstrates that the combined use of SEM-CL imaging and LA-ICP-MS anal-yses, coupled with fluid inclusion microthermometry, can constrain the different fluid conditions of ore forming and the barren stages of evolving submarine hydrothermal systems.