TY - JOUR

T1 - Mass spectrometry based analysis of nucleotides, nucleosides, and nucleobases-application to feed supplements

AU - Neubauer, Stefan

AU - Rugova, Ariana

AU - Chu, Dinh Binh

AU - Drexler, Hedda

AU - Ganner, Anja

AU - Sauer, Michael

AU - Mattanovich, Diethard

AU - Hann, Stephan

AU - Koellensperger, Gunda

N1 - Funding Information: Acknowledgements This work was supported by the Biomin B.R.A.I.N program and by the Austrian Research Promotion Agency FFG (FHplus projekt METORGANIC).

PY - 2012/8

Y1 - 2012/8

N2 - In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100 % aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection <1 μmol L1 could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.

AB - In this work, accurate MS-based methods for quantitative profiling of nucleotides, nucleosides, and nucleobases in yeast extracts used as additives in animal feedstuff are presented. Reversed-phase chromatography utilizing a stationary phase compatible with 100 % aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ion-pair reversed-phase separation. The novel separation method was combined with both molecular and elemental mass spectrometry. By use of RP-LC-MS-MS, excellent limits of detection <1 μmol L1 could be obtained for all the compounds investigated. The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection. Sensitivity of LC-ICP-MS was 1-2 orders of magnitude lower than that of LC-MS-MS. Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement.

KW - Feed supplemen

KW - LC-ICP-MS

KW - LC-MS-MS

KW - Nucleobase

KW - Nucleoside

KW - Nucleotide

UR - http://www.scopus.com/inward/record.url?scp=84865497284&partnerID=8YFLogxK

U2 - 10.1007/s00216-012-6170-9

DO - 10.1007/s00216-012-6170-9

M3 - Article

C2 - 22752403

AN - SCOPUS:84865497284

VL - 404.2012

SP - 799

EP - 808

JO - Analytical and bioanalytical chemistry

JF - Analytical and bioanalytical chemistry

SN - 1618-2642

IS - 3

ER -